HPLC answers 'how much of this is one thing?'
Reverse-phase high-performance liquid chromatography separates a sample by hydrophobicity. The detector records everything that elutes, and purity is expressed as the area of the main peak relative to total peak area.
It is an excellent purity measure and a poor identity measure — a structurally similar impurity can co-elute and inflate the number.
Mass spectrometry answers 'what is this?'
Mass spectrometry ionises the sample and measures mass-to-charge ratio, giving a molecular mass that can be compared against the theoretical mass calculated from the sequence.
Agreement between observed and theoretical mass is the strongest routine evidence that the vial contains the sequence claimed on the label.
Why both are mandatory
Together they answer both questions: mass spectrometry establishes what the material is, HPLC establishes how much of the sample is that material. Aura Peptide Co. releases nothing on a single technique.
Research use only
All materials referenced here are supplied by Aura Peptide Co. strictly for in-vitro laboratory and research use. Nothing on this page is guidance for human or animal use.